This manual on the course "Nanobiotechnology" was created by the Chair of Biophysics and Bioengineering(the Biological Faculty of the Lomonosov Moscow State University). It includes description of modern instruments (atomic force microscopy, confocal microscopy, laser interference microscopy, Raman spectroscopy and EPR) and modeling techniques, as well as laboratory work cycle devoted to the use of nanostructures (quantum dots, colloidal particles, liposomes).